Search results for "antioxidant enzymes"

showing 10 items of 14 documents

Safflower Yellow and Its Main Component HSYA Alleviate Diet-Induced Obesity in Mice: Possible Involvement of the Increased Antioxidant Enzymes in Liv…

2020

PurposeOxidative stress plays an important role in the pathogenesis of obesity and its associated disorders. Safflower yellow (SY) and hydroxysafflor yellow A (HSYA), the natural compounds isolated from Carthamus tinctorius L., has been found to possess antioxidative and anti-obesity properties. The purpose of the present study is to investigate whether SY and its main component HSYA alleviate obesity by the antioxidant effects.MethodsDiet-induced obese (DIO) mice were treated with 200 mg/kg/d SY or HSYA for 10 weeks. Body weight, fat mass, serum biochemical parameters and superoxide dismutase (SOD) activities were measured. Glucose and insulin tolerance tests were performed. The expression…

0301 basic medicinemedicine.medical_specialtyobesityAntioxidantmedicine.medical_treatmentAdipose tissueCarbohydrate metabolismmedicine.disease_causeliverSuperoxide dismutase03 medical and health sciences0302 clinical medicineantioxidant enzymesInternal medicinemedicinePharmacology (medical)Original ResearchPharmacologysafflower yellow (SY)biologyChemistryCarthamuslcsh:RM1-950Metabolismbiology.organism_classificationadipose tissue030104 developmental biologyEndocrinologylcsh:Therapeutics. Pharmacology030220 oncology & carcinogenesishydroxysafflor yellow A (HSYA)biology.proteinLiver functionOxidative stressFrontiers in Pharmacology
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Oxidative stress and antioxidant response in fibroblasts from Werner and Atypical Werner Syndromes

2014

Werner Syndrome (WS, ICD-10 E34.8, ORPHA902) and Atypical Werner Syndrome (AWS, ICD-10 E34.8, ORPHA79474) are very rare inherited syndromes characterized by premature aging. While approximately 90% of WS individuals have any of a range of mutations in theWRN gene, there exists a clinical subgroup in which the mutation occurs in the LMNA/C gene in heterozygosity. Although both syndromes exhibit an age-related pleiotropic phenotype, AWS manifests the onset of the disease during childhood, while major symptoms in WS appear between the ages of 20 and 30. To study the molecular mechanisms of progeroid diseases provides a useful insight into the normal aging process. Main changes found were the d…

AdultMalePremature agingAgingWerner Syndrome HelicaseAdolescentBiologymedicine.disease_causeAntioxidantsCell LineWerner Syndrome HelicaseLMNAProgeriaSuperoxide Dismutase-1antioxidant enzymesmedicineoxidative stressHumansRNA MessengerAtypical Werner syndromeChildeducationCell ProliferationWerner syndromeeducation.field_of_studyProgeriaAtypical Werner SyndromeRecQ Helicasespremature agingSuperoxide DismutaseAging PrematurethioredoxinglutaredoxinCell BiologyFibroblastsLamin Type Amedicine.diseaseGlutathioneMolecular biologyExodeoxyribonucleasesCase-Control StudiesMutationDNA damageFemaleWerner SyndromeThioredoxinOxidative stressResearch PaperAging
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Lifelong soya consumption in males does not increase lifespan but increases health span under a metabolic stress such as type 2 diabetes mellitus.

2021

Soya consumption can decrease oxidative stress in animal models. Moreover, phytoestrogens such as genistein, present in soya, can mimic some of the beneficial effects of estrogens and are devoid of significant side effects, such as cancer. In this study, we have performed a controlled lifelong study with male OF1 mice that consumed either a soya-free diet or a soya-rich diet. We show that, although we found an increase in the expression and activity of antioxidant enzymes in soya-consuming mice, it did not increase lifespan. We reasoned that the soya diet could not increase lifespan in a very healthy population, but perhaps it could extend health span in stressed animals such as type 2 diab…

Agingmedicine.medical_specialtyAntioxidantGoto Kakizaki ratsmedia_common.quotation_subjectmedicine.medical_treatmentLongevityGenisteinPhytoestrogensDiseasemedicine.disease_causeAntioxidants03 medical and health scienceschemistry.chemical_compoundfluids and secretions0302 clinical medicineStress PhysiologicalInternal medicineMedicineAnimals030304 developmental biologymedia_common2. Zero hunger0303 health sciencesbusiness.industryLongevityfood and beveragesType 2 Diabetes MellitusIsoflavonesAnimal FeedIsoflavones3. Good healthRatsOxidative StressEndocrinologychemistryDiabetes Mellitus Type 2Soya030220 oncology & carcinogenesisAntioxidant enzymesPhytoestrogensSoybeansbusinessOxidative stressDevelopmental BiologyMechanisms of ageing and development
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Phytochemical profile and antioxidative properties of Plinia trunciflora fruits: A new source of nutraceuticals.

2020

Abstract This study evaluated the polyphenol profile and the antioxidative properties of Plinia trunciflora (O. Berg) Kausel fruits. Folin-Ciocalteau and pH-jumping methods indicated that these berries are a major source of antioxidant polyphenols (1201.05 mg GAE/100 g FW), particularly anthocyanins. HPLC-DAD-ESI-MS/MS analysis identified cyanidine glycosides as the main components. Flavon-3-ols and hydrolysable-tannins were also found. CAA assay showed that extracts of P. trunciflora fruits prevent lipid peroxidation in HepG2 cells with higher efficacy than other colourful fruits (CAA50 935.25 mg FW/mL cell medium). Moreover, our results suggested that the observed antioxidant protection i…

AntioxidantDPPHmedicine.medical_treatmentMyrtaceaePhytochemicals01 natural sciencesAntioxidantsAnalytical ChemistryAnthocyaninsLipid peroxidationchemistry.chemical_compoundTandem Mass SpectrometrySettore BIO/10 - BiochimicaFood scienceChromatography High Pressure LiquidChromatographyABTSbiologyChemistryfood and beverages04 agricultural and veterinary sciencesGeneral MedicineHep G2 CellsJaboticaba040401 food scienceUp-RegulationPhytochemicalHigh Pressure LiquidAntioxidant enzymesMyrciaria truncifloraPlinia0404 agricultural biotechnologyNutraceuticalmedicineHumansGlutathione PeroxidasePlant ExtractsSuperoxide DismutaseAntioxidant enzyme010401 analytical chemistryPolyphenolsbiology.organism_classification0104 chemical sciencesPolyphenolFruitDietary SupplementsLipid PeroxidationAnthocyanins; Antioxidant enzymes; Cellular antioxidant activity (CAA); Jaboticaba; Myrciaria trunciflora; Antioxidants; Chromatography High Pressure Liquid; Dietary Supplements; Fruit; Glutathione Peroxidase; Hep G2 Cells; Humans; Lipid Peroxidation; Myrtaceae; Phytochemicals; Plant Extracts; Polyphenols; Superoxide Dismutase; Tandem Mass Spectrometry; Up-RegulationCellular antioxidant activity (CAA)Food ScienceFood chemistry
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Profiling the physiological and molecular response to sulfonamidic drag in Procambarus clarkii

2014

Sulfamethoxazole (SMZ) is one of the most widely employed sulfonamides. Because of the widespread use of SMZ, a considerable amount is indeed expected to be introduced into the environment. The cytotoxicity of SMZ relies mainly on arylhydroxylamine metabolites (S-NOH) of SMZ and it is associated with the production of reactive oxygen species (ROS). There is limited information about the toxic potential of SMZ at the cellular and molecular levels, especially in aquatic and/or non-target organisms. In the present study, the red swamp crayfish (Procambarus clarkii), being tolerant to extreme environmental conditions and resistant to disease, was used as a model organism to profile the molecula…

Blood GlucoseGillsHemocytesAntioxidantSulfamethoxazolePhysiologyHealth Toxicology and Mutagenesismedicine.medical_treatmentHepatopancreasHaemolymphatic parametersAquacultureAstacoideaToxicologyBiochemistryArthropod ProteinsAnti-Infective AgentsRed swamp crayfishStress PhysiologicalGene expressionmedicineAnimalsMetallothioneinHSP70 Heat-Shock ProteinsTissue DistributionAntiossidanti enzymesharmony patio parameters proinflammatory genes red swap crac fish sulfametoxazoleProcambarus clarkiichemistry.chemical_classificationReactive oxygen speciesbiologyGene Expression Regulation DevelopmentalProinflammatory genesCell BiologyGeneral Medicinebiology.organism_classificationBlood Cell CountHsp70FerritinBiochemistrychemistryMolecular ResponseFerritinsbiology.proteinMetallothioneinAntioxidant enzymesOxidoreductasesBiomarkersWater Pollutants Chemical
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Sensitivity of liver metabolism in jerboa (Jaculus orientalis) to ciprofibrate, a peroxisome proliferator.

2009

International audience; Ciprofibrate is a well-known drug used to normalize lipid parameters and fibrinogen in atherosclerosis patients. In laboratory rodents such as rats or mice, ciprofibrate exhibits peroxisome proliferator activity. However, to date, no clear alterations or side effects caused by ciprofibrate have been noted in humans. In order to further investigate such possible relationships, we studied the effects of sustained ciprofibrate treatment in jerboas (Jaculus orientalis). In these rodents, ciprofibrate does not induce hepatomegaly or promote liver cell DNA replication, confirming that this species more closely resembles humans than do rats or mice. The jerboas were treated…

Cancer Researchmedicine.medical_specialtyD-3-hydroxybutyrate dehydrogenaseDehydrogenaseBiochemistryJaculus orientalischemistry.chemical_compoundciprofibrateantioxidant enzymesInternal medicineGeneticsmedicine[SDV.BBM] Life Sciences [q-bio]/Biochemistry Molecular Biology[SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biologyclinical enzymesMolecular Biology[ SDV.BBM ] Life Sciences [q-bio]/Biochemistry Molecular Biologysubcellular markerbiologyLiver cellPeroxisomeMalondialdehydeEndocrinologyOncologychemistryCatalasebiology.proteinKetone bodiesMolecular MedicineNAD+ kinaseCiprofibratemedicine.drug
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Exercise and antioxidant supplements in the elderly

2013

Abstract Both exercise and aging increase reactive oxygen species (ROS), which can result in damage to cells. Aging is the result of damage caused by ROS to the mitochondrial genome in post mitotic cells and numerous studies have demonstrated an increase in ROS or their byproducts with exercise. ROS can cause oxidative stress as they overwhelm the antioxidant cellular defenses. Therefore interventions aimed at limiting or inhibiting ROS production, such as supplementation with antioxidant vitamins, should be able to reduce fatigue during muscle contraction and the rate of formation of aging changes with a consequent reduction of the aging rate and disease pathogenesis. However, it has been …

Cell signalingAgingAntioxidantmedicine.medical_treatmentmedia_common.quotation_subjectPopulationPGC-1αSkeletal musclePhysical Therapy Sports Therapy and RehabilitationPharmacologyBiologymedicine.disease_causeNF-κBchemistry.chemical_compoundmedicineOrthopedics and Sports Medicineeducationmedia_commonchemistry.chemical_classificationeducation.field_of_studyReactive oxygen speciesAdaptationsLongevitySkeletal muscleNF-κBmedicine.anatomical_structurechemistryBiochemistryOxidative stressAntioxidant enzymesOxidative stressJournal of Sport and Health Science
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Lafora disease fibroblasts exemplify the molecular interdependence between thioredoxin 1 and the proteasome in mammalian cells

2013

13 páginas, 8 figuras (que no aparecen en este documento, se pueden consultar en: http://www.sciencedirect.com/science/article/pii/S0891584913003274#ec0005)

Cell signalingProteasome Endopeptidase ComplexBlotting WesternFree radicalsBiologyBiochemistryLafora diseaseThioredoxin 1MiceThioredoxinsPhysiology (medical)medicineAnimalsHumansImmunoprecipitationLafora diseaseEndoplasmic Reticulum Chaperone BiPCell proliferationMicroscopy ConfocalProteasomeReverse Transcriptase Polymerase Chain ReactionEndoplasmic reticulumCell cycleFibroblastsSubcellular localizationmedicine.diseaseFlow CytometryCell biologyRare diseasesCytosolOxidative StressBiochemistryProteasomeLafora DiseaseUnfolded protein responseNIH 3T3 CellsAntioxidant enzymesOxidation-Reduction
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Hepatic and branchial xenobiotic biomarker responses in Solea spp. from several NW Mediterranean fishing grounds

2015

9 pages, 4 figures, 4 tables

GillsMaleCommon soleGillInsecticidesDiazinonGlutathione reductaseZoologyAquatic ScienceBiologyOceanographyXenobioticschemistry.chemical_compoundFlatfishSpecies SpecificityMediterranean SeaAnimalsTissue DistributionCbEGSTchemistry.chemical_classificationMusclesGlutathione peroxidasefungiEnvironmental ExposureGeneral MedicineEnvironmental exposurebiology.organism_classificationPollutionLiverchemistryBiochemistrySpainDiazinonDichlorvosFlatfishesFemaleERODAntioxidant enzymesXenobioticBiomarkersWater Pollutants ChemicalEnvironmental Monitoring
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Bioactive Compounds and Antioxidant Properties with Involved Mechanisms of Eugenia involucrata DC Fruits

2022

In this study, the phytochemical profile and the antioxidative properties of Eugenia involucrata fruits were evaluated. Spectrophotometric assays indicated that these berries are a rich source of polyphenols with very high radical-scavenging and metal-reducing activities. High-performance liquid chromatography–Orbitrap analysis was able to carry out the annotation of 36 different compounds, mainly belonging to the flavonol, flavan-3-ol, and anthocyanin families. Antioxidant activity of the fruit extract was evaluated in a cell-based lipid peroxidation model. Obtained data showed that the extract, at very low concentration, was able to prevent oxidative damage in HepG2 cells exposed to oxida…

HPLC-OrbitrapPhysiologyABTS; CAA; Cereja-do-Rio-Grande; HPLC-Orbitrap; anthocyanins; antioxidant enzymes; gene expression; oxidative stressClinical BiochemistryCell BiologyBiochemistryanthocyaninsCereja-do-Rio-Grandeantioxidant enzymesSettore BIO/10 - Biochimicagene expressionoxidative stressABTSMolecular BiologyCAA
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